成功率97%以上で8時間連続運用可能な無人hERGスクリーニング

スタッカと温度制御を備えたQube 384と、より優れたhERG細胞株を使用すると、8時間以上の無人クリーニング実験が可能になります。得られたデータの品質チェックも自動で行われるの97%以上の有用な結果を得ることができます。高い成功率に加えて、Z値は0.5を下回ることはなく、平均で0.76を示しています。このことは、Qubeが非常に大きいスクリーン処理能力を持っていることの証拠です。

無人クリーニングの実施中は、ステッカーがQチップと化合物プレートを自動供給し、温度制御機能が実験中の細胞周囲において安定した温度(本実験では25℃設定)を保証します。

より詳しい情報は、こちらから

Qubeによって解明された作用メカニズム

最近、作用メカニズムの研究に関する2報の論文が発表されました。Xenon社とサイモン・フレイザー大学による電位依存性ナトリウム電流に対するカンナビジオールの抑制効果の論文では、電位依存性ナトリウムチャネルへのカンナビジオールの結合力とメカニズムを解明するためにQubeの温度コントロール機能が使用されました。興味深いことに、結合力は低い温度の方がより高まることが明らかになりました。また、Xenon社とGenentech社の共同研究による選択的Nav1.7アンタゴニストに関する論文では、炎症性および神経因性疼痛に関連するNav1.7の選択的抑制を示す数種の化合物のメカニズムについて論述しています。これらの化合物は非常にゆっくりと作用しますが、Qubeは非常に良好な電圧制御を保ちつつ、1時間以上の長時間にわたって実験を行うことができます。この点に関して著者は論文の中で以下のとおり述べています。

「最小シール抵抗と最小電流サイズを設定したフィルタが適切に使用し、80%以上のシリーズ抵抗を補償することで、マニュアルパッチクランプと同等の高品質なナトリウムチャネル電流の記録が得られる。」

創薬研究向けに開発されたQube

あるお客様に尋ねました,いったいどうやって数十万個もの化合物を,高い忠実度を保った電気生理学的手法でスクリーニングするのかと.すると,以下の応えが返ってきました.

うちではQubeを使って夜間に大規模なスクリ-ニング試験を無人で行っているよ.だから昼間は同じシステムで別の試験を走らせたり,新たな試験の立ち上げができるんだ.”

Juha Kammonen, Principal Scientist, Charles River Laboratories

 

hCav1.2 recordings using QPatch

The development of screening assays for the CaV1.2 channel has been challenging due to the tendency of the channel to exhibit declining current levels (rundown) during the experiment. Here we report about a robust CaV1.2 QPatch assay yielding high success rates, low rundown and reliable pharmacology.

  • Pharmacology and current-voltage relationship in accordance with literature values
  • Success rates of up to 98%
  • Stable currents with rundown as low as 2% per minute
  • For the full application report please see here.

ICMS 2018 coming up shortly…..

We look very much forward to seeing so many people again in Cambridge for the third Ion Channel Modulation Symposium. All the tickets have been sold out for quite a while, but you can still register on our waiting list – there may be cancellations.

You can see the full agenda here. Please click here if you’d like to see some of the great talks from last years’ ICMS.

GABAA pharmacology on cell lines and primary astrocytes on QPatch

In this new Sophion Application report  GABA receptor pharmacology was evaluated on QPatch. We did thorough compound evaluations in a GABAA(α5β3γ2) cell line and investigated the GABA response of primary hippocampal rat astrocytes with emphasis on:

  • Effects of agonists, antagonists, and modulators
  • Concentration-response relationships
  • EC50 and IC50 determination
  • Characterizing both the pharmacology of a specific isolated GABAA subtype and the physiological GABA response of cultured rat astrocytes

Read the full report here.

Also, you are welcome to check out our other publications on GABA here.

Cor.4U human iPS cell-derived cardiomyocytes on QPatch and Qube

Two new application reports with Cor.4U® human iPS cell-derived cardiomyocytes from Ncardia on QPatch and Qube.

The hiPSC – CM provide new strategies to assess cardiotoxicity in vitro and different technologies are available to assess compound effects on cardiomyocytes.

Here we demonstrate

  • High throughput and high fidelity voltage and current clamp recordings
  • Presence of INa, ICa and IKr
  • Paced and spontaneous action potentials
  • We also show that the use of fluoride in the internal solution resulted in lower ICa and shortened action potential durations on QPatch.

See the report for QPatch (Link) and for Qube (Link)

Also, you are welcome to check out our other publications on stem cells here

How easy is it to use Qube?

At Sophion we believe that ease of use is important. When a customer spontaneously express clear appreciation of how easy it is to use our systems, we know we got it right:

“It was true that it’s really up and running after two days training. We are very pleased”

Maija Ivaska, Research Assistant, Orion Pharma

Ease of use does not come by itself with sophisticated electrophysiological recordings in automated patch clamp, but is embedded in the Sophion DNA.

Ask for a demo anywhere in the world to experience this yourself. We are so confident that you will be allowed to run your own experiments at the end of the first day….and that is not always the case with APC instruments.

With our instruments you can perform experiments with a very low risk of human error and large degree standardization, enabling easy learning, many operators and high laboratory efficiency.

Read more about Qube here.

Safety Pharmacology Society Annual Meeting 2018

We look forward to seeing you in Washington D.C. for the annual SPS meeting.

Venue:

Marriott Wardman Park
2660 Woodley Rd NW,
Washington, DC 20008

Come and see us for an ion channel talk at booth #113. Read more about the event here.

 

Channelopathy 2018

Join us and 180 peers in this international conference on ion channel diseases with emphasis on sharing the latest discoveries and technologies.

Venue: Feinberg School of Medicine in Chicago

Read more about the event here.

Neuroscience 2018

Hope to see you in San Diego in November for the annual SfN meeting. You can find us at booth #1933. Want to know more about Neuroscience click here.

Much more information about our activities during the event will be posted.