包括的自動パッチクランプ
自動パッチクランプをツールパックとして所有する研究所が増えています。研究成果と早いフィードバックに注目が集まる中、従来の手動型パッチクランプに加え、自動パッチクランプの価値が高まっています。
－ 数百〜数千倍のデータスループット
－ ユーザースキルに依存しない簡単操作
－ 学術研究機関を上回るテクニカルナレッジ
－ 細胞株、幹細胞、プライマリ細胞に適用可能
「電気生理学者の間では、いまだにベンチマークとしてMPCが行われていますが、多数のトレーニングと技術的な経験を必要とすることがデメリットです。これは、成果と研究プログラムの蓋然性が注目されている学術研究機関で、ますます問題になっています。」
(Bell and Dallas 2018)
あなたの研究室に未来を
私たちは、研究者が機器の操作に時間を費やすのではなく、優れた研究の実現に時間を費やしてほしいと考えています。
そのためには、最先端の測定ソリューションでも使いやすく、多くのユーザー用に設計された高い耐久性を備えている機器が必要です。Sophionのシステムがリリースされ、10年～15年経ちますが問題なく機能しています。システムの操作も10分ほどで簡単に習得できるため、結果の分析や次の新たな研究に時間を費やすことができます。
しかし予算の心配もありますよね？このシステムは10人～15人の熟練した電気生理学者に匹敵する仕事を簡単にこなしますが、価格はその人件費には及びません。ぜひお問い合わせください。驚くことでしょう。
－ 装置や機器の操作ではなく研究に時間を費やす
－ より多くのデータポイントと反復で研究スピードを上げる
－ 製薬業界の将来の可能性を博士や研究員に提供する
－ 耐久性のある研究環境を確立する
中核研究室 – 共有は思いやり
過去のAPC機器への投資は、製薬会社と少数のアカデミックグループにのみ限られていましたが、中核研究施設で機器を共有することが徐々に主流となっています。
－ 部門間で技術の共有
－ 部門間で知識の共有
－ 部門間でコストの分割
「学界で採用されているモデルの１つは、複数の研究グループで資金を提供し、必要な実験を生む中核施設です。」
(Bell and Dallas 2018)
科学的価値 – それは間違いない
「一枚の絵は千以上のことを語る」論文全般にも同じことが言えます。
QPatchやQube384を使用している学術機関発行の出版物リストは、
以下を参照してください。
APCソリューションについては、こちらをクリ
ックしてください – QPatch IIとQube 384
出版物リスト（学術機関より）
– 包括リストについては 公表文献など を参照
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Agwa, Akello J. et al. 2020. “Manipulation of a Spider Peptide Toxin Alters Its Affinity for Lipid Bilayers and Potency and Selectivity for Voltage-Gated Sodium Channel Subtype 1.7.” Journal of Biological Chemistry 295(15): 5067–80. https://www.jbc.org/content/early/2020/03/05/jbc.RA119.012281.full.pdf.
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Al-Sabi, Ahmed et al. 2017. “A Rational Design of a Selective Inhibitor for Kv1.1 Channels Prevalent in Demyelinated Nerves That Improves Their Impaired Axonal Conduction.” Journal of Medicinal Chemistry 60(6): 2245–56. https://doi.org/10.1021/acs.jmedchem.6b01262.
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Bagchi, Bandita et al. 2014. “Disruption of Myelin Leads to Ectopic Expression of KV1.1 Channels with Abnormal Conductivity of Optic Nerve Axons in a Cuprizone-Induced Model of Demyelination.” PLoS ONE 9(2).
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Billakota, Santoshi et al. 2019. “Personalized Medicine: Vinpocetine to Reverse Effects of GABRB3 Mutation.” Epilepsia 60(12): 2459–65.
Billet, Arnaud, Lionel Froux, John W. Hanrahan, and Frederic Becq. 2017. “Development of Automated Patch Clamp Technique to Investigate CFTR Chloride Channel Function.” Frontiers in Pharmacology 8(APR): 1–10.
Brown, Maile R. et al. 2016. “Physiological Modulators of Kv3.1 Channels Adjust Firing Patterns of Auditory Brain Stem Neurons.” Journal of Neurophysiology 116(1): 106–21.
Cardoso, Fernanda C. et al. 2015. “Identification and Characterization of ProTx-III [μ-TRTX-Tp1a], a New Voltage-Gated Sodium Channel Inhibitor from Venom of the Tarantula Thrixopelma Pruriens.” Molecular Pharmacology 88(2): 291–303.
Choi, Ryan et al. 2020. “Bumped Kinase Inhibitors as Therapy for Apicomplexan Parasitic Diseases: Lessons Learned.” International Journal for Parasitology. http://www.sciencedirect.com/science/article/pii/S0020751920300564.
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Das, Pragnya et al. 2019. “Novel Chitohexaose Analog Protects Young and Aged Mice from CLP Induced Polymicrobial Sepsis.” Nature Scientific Reports 9(1): 1–12.
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Deuis, Jennifer R. et al. 2016. “Analgesic Effects of GpTx-1, PF-04856264 and CNV1014802 in a Mouse Model of NaV1.7-Mediated Pain.” Toxins 8(3): 1–19.
Deuis, Jennifer R. et al. 2017. “Pharmacological Characterisation of the Highly Na v 1.7 Selective Spider Venom Peptide Pn3a.” Nature Scientific Reports 7(January): 1–19.
Diness, Jonas Goldin et al. 2017. “Termination of Vernakalant-Resistant Atrial Fibrillation by Inhibition of Small-Conductance Ca2+-Activated K+ Channels in Pigs.” Circulation: Arrhythmia and Electrophysiology 10(10): 1–13.
Flinspach, M. et al. 2017. “Insensitivity to Pain Induced by a Potent Selective Closed-State Nav1.7 Inhibitor.” Nature Scientific Reports 7(January): 1–16.
Franz, Denise, Hervør Lykke Olsen, Oliver Klink, and Jan Gimsa. 2017. “Automated and Manual Patch Clamp Data of Human Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons.” Nature Scientific Data 4: 1–11.
Ghovanloo, Mohammad Reza et al. 2019. “Inhibitory Effects of Cannabidiol on Voltage-Dependent Sodium Currents.” Journal of Biological Chemistry 293(43): 16546–58.
Grillo, Alessandro et al. 2019. “Development of Novel Multipotent Compounds Modulating Endocannabinoid and Dopaminergic Systems.” European Journal of Medicinal Chemistry 183: 111674. https://doi.org/10.1016/j.ejmech.2019.111674.
Hammami, Sofia et al. 2009. “Cell Volume and Membrane Stretch Independently Control K+ Channel Activity.” Journal of Physiology 587(10): 2225–31.
Hao, Yuchen, Jingshu Tang, and KeWei Wang. 2015. “Development of Automated Patch Clamp Assay for Evaluation of Α7 Nicotinic Acetylcholine Receptor Agonists in Automated QPatch-16.” ASSAY and Drug Development Technologies 13(3): 174–84. https://doi.org/10.1089/adt.2014.622.
He, Linhong et al. 2018. “Design, Synthesis and Biological Evaluation of 7H-Pyrrolo[2,3-d]Pyrimidin-4-Amine Derivatives as Selective Btk Inhibitors with Improved Pharmacokinetic Properties for the Treatment of Rheumatoid Arthritis.” European Journal of Medicinal Chemistry 145: 96–112. http://www.sciencedirect.com/science/article/pii/S0223523417311108.
Hirsch, Rolf et al. 2020. “Antimicrobial Peptides from Rat-Tailed Maggots of the Drone Fly Eristalis Tenax Show Potent Activity against Multidrug-Resistant Gram-Negative Bacteria.” Microorganisms 8(5): 1–20.
Inserra, Marco C. et al. 2017. “Multiple Sodium Channel Isoforms Mediate the Pathological Effects of Pacific Ciguatoxin-1.” Nature Scientific Reports 7(January): 1–19.
Israel, Mathilde R et al. 2018. “The E15R Point Mutation in Scorpion Toxin Cn2 Uncouples Its Depressant and Excitatory Activities on Human NaV1.6.” Journal of Medicinal Chemistry 61(4): 1730–36. https://doi.org/10.1021/acs.jmedchem.7b01609.
Jalily, Pouria H. et al. 2020. “Put a Cork in It: Plugging the M2 Viral Ion Channel to Sink Influenza.” Antiviral Research 178(February): 104780. https://www.sciencedirect.com/science/article/pii/S0166354219305820.
Jenkins, David Paul et al. 2013. “Development of a QPatch Automated Electrophysiology Assay for Identifying KCa3.1 Inhibitors and Activators.” Assay and Drug Development Technologies 11(9–10): 551–60.
Jin, Ai Hua et al. 2015. “δ-Conotoxin SuVIA Suggests an Evolutionary Link between Ancestral Predator Defence and the Origin of Fish-Hunting Behaviour in Carnivorous Cone Snails.” Proceedings of the Royal Society B: Biological Sciences 282(1811).
Kaproń, Barbara et al. 2019. “Development of the 1,2,4-Triazole-Based Anticonvulsant Drug Candidates Acting on the Voltage-Gated Sodium Channels. Insights from in-Vivo, in-Vitro, and in-Silico Studies.” European Journal of Pharmaceutical Sciences 129: 42–57. http://www.sciencedirect.com/science/article/pii/S0928098718305530.
Klint, Julie K., Yanni K.Y. Chin, and Mehdi Mobli. 2015. “Rational Engineering Defines a Molecular Switch That Is Essential for Activity of Spider-Venom Peptides against the Analgesics Target NaV1.7.” Molecular Pharmacology 88(6): 1002–10.
Kramer, James et al. 2013. “MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes.” Nature Scientific Reports 3: 1–7.
Kramer, James et al. 2020. “Cross-Site and Cross-Platform Variability of Automated Patch Clamp Assessments of Drug Effects on Human Cardiac Currents in Recombinant Cells.” Nature Scientific Reports 10(1): 1–15. http://dx.doi.org/10.1038/s41598-020-62344-w.
Kristof, Arnold S. et al. 2017. “An Official American Thoracic Society Workshop Report: Translational Research in Rare Respiratory Diseases.” Annals of the American Thoracic Society 14(8): 1239–47.
Łażewska, Dorota et al. 2017. “Biphenyloxy-Alkyl-Piperidine and Azepane Derivatives as Histamine H3 Receptor Ligands.” Bioorganic & Medicinal Chemistry 25(20): 5341–54. http://www.sciencedirect.com/science/article/pii/S0968089617310696.
Liao, Weike et al. 2020. “Design, Synthesis and Biological Activity of Novel 2,3,4,5-Tetra-Substituted Thiophene Derivatives as PI3Kα Inhibitors with Potent Antitumor Activity.” European Journal of Medicinal Chemistry 197: 112309. https://doi.org/10.1016/j.ejmech.2020.112309.
Loucif, Alexandre J.C. et al. 2018. “GI-530159, a Novel, Selective, Mechanosensitive Two-Pore-Domain Potassium (K 2P ) Channel Opener, Reduces Rat Dorsal Root Ganglion Neuron Excitability.” British Journal of Pharmacology 175(12): 2272–83.
Marcinkowska, Monika et al. 2016. “Design, Synthesis, and Biological Evaluation of Fluorinated Imidazo[1,2-a]Pyridine Derivatives with Potential Antipsychotic Activity.” European Journal of Medicinal Chemistry 124: 456–67. http://www.sciencedirect.com/science/article/pii/S0223523416307152.
Menegon, A. et al. 2017. “A New Electro-Optical Approach for Conductance Measurement: An Assay for the Study of Drugs Acting on Ligand-Gated Ion Channels.” Nature Scientific Reports 7(September 2016): 1–13.
Miner, Kent et al. 2019. “Drug Repurposing: The Anthelmintics Niclosamide and Nitazoxanide Are Potent TMEM16A Antagonists That Fully Bronchodilate Airways.” Frontiers in Pharmacology 10(FEB).
Mistry, Hitesh B., Mark R. Davies, and Giovanni Y. Di Veroli. 2015. “A New Classifier-Based Strategy for in-Silico Ion-Channel Cardiac Drug Safety Assessment.” Frontiers in Pharmacology 6(MAR): 1–6.
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Nausch, Bernhard et al. 2014. “NS19504: A Novel BK Channel Activator with Relaxing Effect on Bladder Smooth Muscle Spontaneous Phasic Contractions.” Journal of Pharmacology and Experimental Therapeutics 350(3): 520–30.
Okada, Jun Ichi et al. 2015. “Screening System for Drug-Induced Arrhythmogenic Risk Combining a Patch Clamp and Heart Simulator.” Science Advances 1(4): 1–8.
Okumu, Antony et al. 2020. “Novel Bacterial Topoisomerase Inhibitors Derived from Isomannide.” European Journal of Medicinal Chemistry 199: 112324. https://doi.org/10.1016/j.ejmech.2020.112324.
Ong, Seow Theng et al. 2019. “Extracellular K + Dampens T Cell Functions: Implications for Immune Suppression in the Tumor Microenvironment.” Bioelectricity 1(3): 169–79.
Petrou, Terry et al. 2017. “Intracellular Calcium Mobilization in Response to Ion Channel Regulators via a Calcium-Induced Calcium Release Mechanism.” Journal of Pharmacology and Experimental Therapeutics 360(2): 378–87.
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Robinson, Samuel D. et al. 2018. “A Comprehensive Portrait of the Venom of the Giant Red Bull Ant, Myrmecia Gulosa, Reveals a Hyperdiverse Hymenopteran Toxin Gene Family.” Science Advances 4(9): 1–13.
Shcherbatko, Anatoly et al. 2016. “Modulation of P2X3 and P2X2/3 Receptors by Monoclonal Antibodies.” Journal of Biological Chemistry 291(23): 12254–70.
Simó-Vicens, Rafel et al. 2017. “Effect of Antiarrhythmic Drugs on Small Conductance Calcium-Activated Potassium Channels.” European Journal of Pharmacology 803: 118–23. http://www.sciencedirect.com/science/article/pii/S0014299917302091.
Singh, Vinayak et al. 2017. “The Inosine Monophosphate Dehydrogenase, GuaB2, Is a Vulnerable New Bactericidal Drug Target for Tuberculosis.” ACS Infectious Diseases 3(1): 5–17.
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Zha, Chuantao et al. 2018. “Design, Synthesis and Biological Evaluation of Tetrahydronaphthyridine Derivatives as Bioavailable CDK4/6 Inhibitors for Cancer Therapy.” European Journal of Medicinal Chemistry 148: 140–53. http://www.sciencedirect.com/science/article/pii/S0223523418301442.
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