12.04.2018

Light activated rubi-GABA on Qube Opto 384

The new optical capability of Qube Opto introduces the possibility of employing optopharmacology in combination with patch clamp in a 384 format. As we continue to challenge our system we will present some of the results here. Recently we have been playing with experiments employing rubi-GABA, a caged GABA compound activated by visual wavelengths.

GABAAR mediated currents was elicited by optical uncaging of GABA in both a concentration and light dependant fashion.

Stay tuned for more news on Qube Opto 384 in the coming weeks

Concentration-response relationship

  • HEK 293 / GABAA (α5β3γ2)
  • Caged GABA (Rubi-GABA – Tocris)
  • Light: 475 nm for 200 ms @ 100% light output intensity
  • Rubi-GABA: 3-fold dilution series
  • EC50: 10,6 uM +/- 0,5 uM

Optical dose-response relationship

  • HEK 293 / GABAA (α5β3γ2)
  • Caged GABA (Rubi-GABA – Tocris)
  • Light: 475 nm for 200 ms @ 10-100% light output intensities
Optical Dose Response relationship. Left: Current traces at 10%, 33% and 100% light intensity. Right: Response as a function of light intensity

For more information on Optogenetic capabilities on Qube Opto 384 contact SWI@sophion.com