Cell culturing for automated patch clamp

著者: Pia B Andersen, Mette T Christensen

Correct cell handling is important for optimal experimental results. This report gives advice on cell handling in order that you can obtain the best cells possible for use on the QPatch or Qube instruments. Here we describe how to culture your cells and how to reach optimal cell confluence for both cell propagation (“mother flasks”) and for experimental use. Detachment enzymes recommended by Sophion are also described together with methods for harvesting and storing cells.