CFTR channels activated by flouride on QPatch

雑誌名: Sophion application report
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著者: Naja Møller Sørensen et al., 2016

Cystic fibrosis transmembrane conductance regulator (CFTR) functions as a chloride channel and controls the regulation of other transport pathways. Mutations in the CFTR gene have been found to cause cystic fibrosis (CF) and congenital bilateral aplasia of the vas deferens (CBAVD).
Here we demonstrate that reliable recordings with the chloride conducting ion channel CFTR can be performed on QPatch using fluoride as activator of adenylate cyclase. We have shown the ability to determine the IC50 for CFTRinh-172 in close correspondence to the literature values.
We can conclude that the overall success in obtaining stable seals and completed experiments are at a level that clearly identifies the CHO–hCFTR assay as feasible and with high quality results on QPatch.