Developing a stable high-throughput assay using electroporation transfected cells

著者: Kim Boddum

Electroporation is a technique allowing chemicals, drugs, or DNA to be introduced into the cell by applying an electrical field to the cells, thereby increasing the permeability of the cell membrane. The Neon® Transfection System is a novel benchtop electroporation device that employs the pipette tip as an electroporation chamber to easily and efficiently transfect mammalian cells.

In this study, native HEK 293 cells were transfected with a plasmid encoding the human glycine receptor α1 (hGlyRα1), to demonstrate how a Qube can be used for:

1. optimization of electroporation transfection

2. pharmacological evaluation of transfected cells with varying transfection levels