GABA-A a17B3y2 on QPatch

著者: Hervor L Olsen, M Knirke Jensen

This report presents QPatch studies based on whole-cell current recordings from the ligand-gated ion channel hGABAA receptors (α1β3γ2), expressed in HEK-293 cells from Millipore. GABAA receptors are anionic channels and upon activation under physiological conditions, an inward chloride driven current is recorded. The channels were targeted with an agonist; GABA (γ-amino-butyric acid) and bicuculline (an antagonist).