HEK293-Cav2.2 on QPatch

It is common that potential customers ask Sophion Bioscience to use the QPatch to test some of their compounds on specific ion channels before buying a system. In this application report, we present some of the results obtained from a validation study done for a big pharmaceutical company. Currents from the N-type voltage gated calcium channels (Ca_v2.2) were studied using the QPatch. A blocker of the Ca_v2.2 current, compound A, was applied in increasing concentrations to study the dose-response effects and estimate IC₅₀ values. Furthermore, flunarizine which is known to block T-type Ca²⁺/Na+ channels, and have low potency on N-type Ca channels, was tested.