High throughput screening for mode-of-action on Nav1.4
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- Constantly high success rates (99% in three experiments)
- Giga-ohm seal resistances in almost all wells
- Very low data spread
- All CV values of DMSO control below 10%
- No false positives with cut-off criteria 3 X SD
- Z’ constantly greater 0.75
- Discrimination between different modes of inhibition (state-dependency)
- Up to 384 individual IC50 values (3072 data points) determined in just one experiment
- Using Qube in combination with stacker it would be possible to determine up to 7680 IC50 (60,000 data points) per 24 hours.