Internal solution exchange on Qube

Internal addition of compounds targeting the chloride channel CIC-1. A robust assay with good pharmacology and high Z-score

• A robust assay of ClC-1 with Z’ constantly >0.5
• Biophysical and pharmacological characteristics as expected for ClC-1
• Qube offers the possibility to apply compounds both intra- and extracellularly
• Advanced analysis of compound effects on channel kinetics are available at a few click

In this report, we show a ClC-1 assay on Qube that shows biophysical characteristics as expected for ClC-1. We further introduce Sophion`s Analyzer software that allows rapid analysis of large data sets to answer advanced electrophysiological questions, in the present case: What is the mode of action of a novel, unknown compound?