心 臓 の イ オ ン チ ャ ネ ル

心 臓 の 活動 電位 は 複数 の イ オ ン チ ャ ネ ル の 出力 に よ っ て 成 り 立 っ て い ま す. 心 臓 の イ オ ン チ ャ ネ ル は, QT 延長 症候群 (LQTS) と 呼 ば れ る 心 臓 血管 疾患 の 標的 で あ り, ま た 薬 物 の 心 臓 安全 性 評 価 の 重要 な 対 象 で もあ り ま す.

 

さまざまなイオンチャネルターゲットにQPatchとQubeで実施された研究に関する出版物、ポスターおよび報告書を以下からご参照ください。

 

Key Cardiac Ion Channels

Kv11.1 (hERG)

Nav1.5

Cav1.2

Kv7.1 (KvLQT/MinK)

Kv4.3

Kv1.5


Kv11.1 (hERG)

Posters

  • 詳細 Simultaneous Measurement of Cardiac hERG and Nav1.5 Currents Using an Automated Qube® Patch Clamp Platform
    Year: 2018 First author: Guo et Al., 2018
  • 詳細 Estimating hERG drug binding using temperature-controlled high throughput automated patch clamp
    Year: 2018 First author: Lindqvist et al. 2018
  • 詳細 Are stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?
    Year: 2017 First author: Morton
  • 詳細 Temperature effect on hERG channel pharmacology measured using the Qube automated patch clamp system
    Year: 2017 First author: Lindqvist
  • 詳細 Qube as a tool for assay optimization of CiPA cells and protocols by using multiple IC, EC solutions and hERG, Nav1.5 and Cav1.2 on the same QChip
    Year: 2016 First author: Schupp et al., 2016
  • 詳細 Enhancing throughput with multiple cell lines per well
    First author: Olsen et al., 2006
  • 詳細 Characterization of hERG blockers using the automated QPatch 16 screening system
    Year: 2005 First author: Jacobsen et al., 2005
  • 詳細 Data quality is not affected by cell suspension density
    Year: 2006 First author: Mathes et al., 2006
  • 詳細 Strategies for enhancing throughput in ion channel drug screening
    Year: 2008 First author: Jacobsen et al., 2008
  • 詳細 Pharmacological characterization of voltage- and ligand-gated ion channels
    Year: 2006 First author: Olsen et al., 2006
  • 詳細 “Clip Detecting” with series resistance compensation using an automated patch clamp system
    Year: 2012 First author: Løjkner et al., 2012
  • 詳細 Optimizing CHO hERG for automated patch clamping
    Year: 2006 First author: Friis et al., 2006
  • 詳細 Verification of CiPA Recommended Voltage Protocols in Patch-Clamp Assay for hERG, Cav1.2 and Late Nav1.5 Currents
    Year: 2019 First author: Katayama et Al., 2019

Papers

  • 詳細 A dipolar cycloaddition reaction to access 6-methyl-4,5,6,7-tetrahydro-1H-[1,2,3]triazolo[4,5-c]pyridines enables the discovery synthesis and preclinical profiling of a P2X7 antagonist clinical candidate
    Year: 2017 First author: Chrovian et Al.
  • 詳細 Discovery and electrophysiological characterization of SKF-32802; a novel hERG agonist found through a large-scale structural similarity search
    Year: 2017 First author: Donovan, Brian T. et Al.
  • 詳細 Synthesis and biological evaluation of novel 6,11-dihydro-5H-benzo[e]pyrimido- [5,4-b][1,4]diazepine derivatives as potential c-Met inhibitors
    Year: 2017 First author: Huang
  • 詳細 MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes
    Year: 2013 First author: Kramer et al., 2013
  • 詳細 Characterization of Potassium Channel Modulators with QPatch™ Automated Patch-Clamp Technology: System Characteristics and Performance To cite this article:
    Year: 2004 First author: Kutchinsky et al., 2004
  • 詳細 Automated Patch Clamping Using the QPatch
    Year: 2009 First author: Jones et al. 2009
  • 詳細 Automated Planar Electrode Electrophysiology in Drug Discovery: Examples of the Use of QPatch in Basic Characterization and High Content Screening on Nav, KCa2.3, and Kv11.1 Channels
    Year: 2009 First author: Korsgaard et al., 2009
  • 詳細 QPatch: the missing link between HTS and ion channel drug discovery.
    Year: 2009 First author: Mathes
  • 詳細 A new classifier-based strategy for in-silico ion-channel cardiac drug safety assessment
    Year: 2015 First author: Mistry et al., 2015
  • 詳細 Screening system for drug-induced arrhythmogenic risk combining a patch clamp and heart simulator
    Year: 2015 First author: Okada et al., 2015
  • 詳細 Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells: An In-Vitro Model to Predict Cardiac Effects of Drugs
    Year: 2017 First author: Sube
  • 詳細 Identification of Quinoline-Based RIP2 Kinase Inhibitors with an Improved Therapeutic Index to the hERG Ion Channel
    Year: 2018 First author: Haile et Al., 2018
  • 詳細 Easy-To-Synthesize Spirocyclic Compounds Possess Remarkable in Vivo Activity against Mycobacterium tuberculosis
    Year: 2018 First author: Guardia et Al., 2018

Reports

  • 詳細 QPatchにおけるCiPA Milnes hERG動態アッセイ
    Year: 2018 First author: Humphries et Al.
  • 詳細 8 hours unattended hERG run with ≥97% success rate and consistent pharmacology results
    Year: 2019 First author: Schupp et Al., 2018
  • 詳細 CHO-hERG Duo characterization and functional validation on QPatch
    Year: 2011 First author: Jacobsen et al., 2011
  • 詳細 CHO-hERG Duo in multi-hole mode on QPatch
    Year: 2011 First author: Jacobsen et al., 2011
  • 詳細 Stable response for ligand and voltage-gated ion channels on QPatch
    Year: 2010 First author: Olsen et al., 2010
  • 詳細 Temperature control on Qube 384 – pharmacological dependency of hERG reference compounds
    Year: 2017 First author: R Jacobsen
  • 詳細 CHO-hERG DUO optimized for QPatch
    Year: 2008 First author: R Jacobsen

Nav1.5

Posters

  • 詳細 Biophysical and pharmacological characterization of multiple Nav subtypes on Qube
    Year: 2016 First author: Lindqvist et Al
  • 詳細 Qube as a tool for assay optimization of CiPA cells and protocols by using multiple IC, EC solutions and hERG, Nav1.5 and Cav1.2 on the same QChip
    Year: 2016 First author: Schupp et al., 2016
  • 詳細 Verification of CiPA Recommended Voltage Protocols in Patch-Clamp Assay for hERG, Cav1.2 and Late Nav1.5 Currents
    Year: 2019 First author: Katayama et Al., 2019
  • 詳細 Electrophysiological properties of iCell cardiomyocytes obtained by automated patch clamp
    Year: 2011 First author: Schrøder et al., 2011
  • 詳細 Use- and state-dependent Nav1.5 blockers on QPatch X and in vivo and in vitro assays
    Year: 2010 First author: Jensen et al., 010
  • 詳細 Rapid generation of cells for ion channels assays: Efficient, large scale transfection using the MaxCyte STX
    First author: Brady et al.
  • 詳細 Enhancing throughput with multiple cell lines per well
    First author: Olsen et al., 2006
  • 詳細 Nav1.5 use-dependent blockers on QPatch X
    Year: 2012 First author: Jacobsen et al., 2012
  • 詳細 Are stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?
    Year: 2017 First author: Morton
  • 詳細 New CiPA cardiac ion channel cell lines and assays for in vitro proarrhythmia risk assessment
    Year: 2018 First author: Humphries et Al., 2018
  • 詳細 Biophysical and pharmacological profiling of multiple voltage-gated sodium channel subtypes on QPatch II
    Year: 2019 First author: Sauter et Al.
  • 詳細 Simultaneous Measurement of Cardiac Nav1.5 Peak and Late Currents in an Automated QPatch Platform
    Year: 2018 First author: Guo et Al., 2018
  • 詳細 Simultaneous Measurement of Cardiac hERG and Nav1.5 Currents Using an Automated Qube® Patch Clamp Platform
    Year: 2018 First author: Guo et Al., 2018
  • 詳細 Hesperetin preferentially inhibits slow-activating currents of an LQT3 syndrome Na+ channel mutation
    Year: 2018 First author: Alvarez-Collazo et Al., 2018

Papers

  • 詳細 Cardiac sodium channel antagonism – Translation of preclinical in vitro assays to clinical QRS prolongation
    Year: 2017 First author: Jenkinson, S.
  • 詳細 Use- and state-dependent Nav1.5 blockers on QPatch X and in vivo and in vitro assays
    Year: 2010 First author: Jensen et al., 010
  • 詳細 Automated Planar Electrode Electrophysiology in Drug Discovery: Examples of the Use of QPatch in Basic Characterization and High Content Screening on Nav, KCa2.3, and Kv11.1 Channels
    Year: 2009 First author: Korsgaard et al., 2009
  • 詳細 MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes
    Year: 2013 First author: Kramer et al., 2013
  • 詳細 A new classifier-based strategy for in-silico ion-channel cardiac drug safety assessment
    Year: 2015 First author: Mistry et al., 2015
  • 詳細 Screening system for drug-induced arrhythmogenic risk combining a patch clamp and heart simulator
    Year: 2015 First author: Okada et al., 2015

Reports

  • 詳細 QPatchを用いたNaV1.5 (late) 電流 心臓安全性評価試験
    Year: 2019 First author: Humphries
  • 詳細 Nav1.5(Late) cardiac safety assay on QPatch
    Year: 2019 First author: Edward S A Humphries
  • 詳細 CHO-Nav1.5 on QPatch
    Year: 2012 First author: Olsen
  • 詳細 Use- and state-dependent Nav1.5 blockers on QPatch X and in vivo and in vitro assays
    Year: 2010 First author: Jensen et al., 010
  • 詳細 Cor.At cells embryonic stem cell derived cardiomyocytes on QPatch
    Year: 2009 First author: Schrøder et al., 2009
  • 詳細 Voltage-gated sodium channels on QPatch 16
    Year: 2012 First author: Olsen 2012

Cav1.2

Posters

  • 詳細 Automated High Throughput Na+ Late Current Assay on QPatch HT Platform for CiPA28
    Year: 2018 First author: Renganathan et Al., 2018
  • 詳細 Verification of CiPA Recommended Voltage Protocols in Patch-Clamp Assay for hERG, Cav1.2 and Late Nav1.5 Currents
    Year: 2019 First author: Katayama et Al., 2019
  • 詳細 Optimization of Cardiac CiPA Targets (hCav1.2 and hKCNQ1/hminK) on the QPatch HT Automated System
    Year: 2018 First author: Koci et Al., 2018
  • 詳細 Qube as a tool for assay optimization of CiPA cells and protocols by using multiple IC, EC solutions and hERG, Nav1.5 and Cav1.2 on the same QChip
    Year: 2016 First author: Schupp et al., 2016
  • 詳細 Exploring stem cell-derived cardiomyocytes with automated patch clamp techniques
    Year: 2012 First author: Schrøder et al., 2012
  • 詳細 Are stem-cell cardiomyocytes a viable cellular reagent for automated patch-clamp?
    Year: 2017 First author: Morton

Papers

  • 詳細 A new classifier-based strategy for in-silico ion-channel cardiac drug safety assessment
    Year: 2015 First author: Mistry et al., 2015
  • 詳細 Screening system for drug-induced arrhythmogenic risk combining a patch clamp and heart simulator
    Year: 2015 First author: Okada et al., 2015
  • 詳細 MICE Models: Superior to the HERG Model in Predicting Torsade de Pointes
    Year: 2013 First author: Kramer et al., 2013

Reports

  • 詳細 hCav1.2 recordings on Qube 384 using step, train and CiPA protocols
    Year: 2018 First author: Schupp
  • 詳細 Cav1.2 on Qube 384
    Year: 2017 First author: Schupp, M
  • 詳細 CHO Cav1.2 on QPatch (ChanTest)
    Year: 2011 First author: Friis et al., 2011
  • 詳細 Cor.At cells embryonic stem cell derived cardiomyocytes on QPatch
    Year: 2009 First author: Schrøder et al., 2009
  • 詳細 Cav1.2 on Qube 384 – pharmacology, stability and CiPA
    Year: 2018 First author: Schupp et Al., 2018

Kv7.1 (KvLQT/MinK)

Posters

  • 詳細 Enhancing throughput with multiple cell lines per well
    First author: Olsen et al., 2006
  • 詳細 Automation of patch clamp techniques: Technical validation through characterization of voltage-gated potassium channels and ligand-gated ion channels
    Year: 2004 First author: Krywkowski et al., 2004

Papers

  • 詳細 Cell volume and membrane stretch independently control K+ channel activity
    Year: 2009 First author: Hammami et al., 2009

Kv4.3

Papers

  • 詳細 Screening system for drug-induced arrhythmogenic risk combining a patch clamp and heart simulator
    Year: 2015 First author: Okada et al., 2015

Kv1.5

Posters

  • 詳細 Enhancing throughput with multiple cell lines per well
    First author: Olsen et al., 2006
  • 詳細 Pharmacological characterization of voltage- and ligand-gated ion channels
    Year: 2006 First author: Olsen et al., 2006

Papers

  • 詳細 Isoindolinone compounds active as Kv1.5 blockers identified using a multicomponent reaction approach
    Year: 2015 First author: Kajanus et al.

Handouts

  • 詳細 CHO-Kv1.5 Voltage-gated potassium channels on QPatch
    Year: 2009 First author: Jensen et al., 2009

HCN

Posters

  • 詳細 Optical modulation of ion channels using Qube Opto
    Year: 2019 First author: Boddum et Al., 2019
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